Journal: RSC Advances

Article Title: Eco-friendly AuNPs@rGO nanocomposite as a peroxidase-mimic for cholesterol detection

doi: 10.1039/d5ra07564e

Figure Lengend Snippet: (a) UV-vis spectra of different ChO concentrations in the AuNPs@rGO + TMB + ChOx system; (b) calibration curve showing a linear range from 0.2–1.2 mM ( R 2 = 0).

Article Snippet: Chemicals such as, cholesterol oxidase (Chox), hydrogen peroxide (H 2 O 2 ), and tetramethylbenzidine (TMB) were purchased from Merck in India.

Techniques:

Journal: Materials Today Bio

Article Title: “Top-down and Bottom-up” cholesterol-depleting biomimetic nanoparticle for enhancing sonodynamic therapy against hepatocellular carcinoma

doi: 10.1016/j.mtbio.2025.102370

Figure Lengend Snippet: A) Cholesterol depletion by HM/ZIF-8@COD/IR820 nanoparticle. B) Catalytic activity of HM/ZIF-8@COD/IR820 nanoparticles at different concentrations, and TMB played as the indicator. C) Relative activity of COD and HM/ZIF-8@COD/IR820 nanoparticle at different pH values (4.5, 5.0, 5.5, 6.0 and 6.5). D) Catalytic activity assay of HM/ZIF-8@COD/IR820 nanoparticle at different time points. E) O 2 generation by different concentrations of nanoparticle (HM/ZIF-8@COD/IR820) at various time points (0, 10, 20, 30, 60, 90, 120, 180, and 240 s). F) Relative catalase activity of RBCM at different pH values. G, H) UV–vis absorption spectra of DPBF in the presence of HM/ZIF-8@COD/IR820 under US irradiation at various time (0, 30, 60, 90, 120, 150 and 180 s) and power intensity (0, 0.30, 0.50, 0.8, 1.0, 1.25, 1.50 and 1.75 W/cm 2 ). I) Intracellular fluorescence images following treatment with RBCM/ZIF-8@COD/IR820 nanoparticle or HM/ZIF-8@COD/IR820 nanoparticle for 0 and 4 h. J) ROS levels quantified by fluorescence imaging under different treatments. Green fluorescence indicates ROS. K) Release of COD and Zn 2+ from HM/ZIF-8@COD/IR820 nanoparticle under different conditions. L, M) Live/dead cell staining and relative cell activity analysis of Hepa1-6 cells following different treatments. Live cells are stained green, while dead cells emit red fluorescence. N) Western blotting analysis of Bax and Bcl-2 expression after various treatments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Cholesterol oxidase (COD), IR820(C 46 H 50 ClN 2 NaO 6 S 2 , MW 849.47), the Filipin complex and Pimonidazole were obtained from MedChemExpress (Shanghai, China).

Techniques: Activity Assay, Irradiation, Fluorescence, Imaging, Staining, Western Blot, Expressing

Journal: Materials Today Bio

Article Title: “Top-down and Bottom-up” cholesterol-depleting biomimetic nanoparticle for enhancing sonodynamic therapy against hepatocellular carcinoma

doi: 10.1016/j.mtbio.2025.102370

Figure Lengend Snippet: A) Proposed therapeutic mechanisms of HM/ZIF-8@COD/IR820 nanoparticle. B) Zinc content in Hepa1-6 cells following different treatments (5, 10, 20, 50 and 100 μg/mL). C, D) Intracellular ATP and NADP + levels after treatment with varying concentrations of HM/ZIF-8@COD/IR820 nanoparticle (5, 10, 15, 25, 50, 75 and 100 μg/mL). E) Cholesterol depletion measured by Filipin staining following different treatments. F) The effect of various treatments on cell migration, evaluated by the wound healing assay. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Article Snippet: Cholesterol oxidase (COD), IR820(C 46 H 50 ClN 2 NaO 6 S 2 , MW 849.47), the Filipin complex and Pimonidazole were obtained from MedChemExpress (Shanghai, China).

Techniques: Staining, Migration, Wound Healing Assay